José António Leonardo dos Santos
Assistant Professor



Research Activities
My current research is focused on plasmid DNA (pDNA) manufacturing processes (production, separation, purification, quality control and monitoring) for application in gene therapy or DNA vaccination. The utilization of membrane processes (ultra and microfiltration) and the development of alternative microbial GRAS (generally recognized as safe) platforms for pDNA production. Lipids, carotenoids and lactic acid, production and separation (biorefinery) are other goals of my research.

Selected Publications
Dias, C., Santos, J.A.L., Reis, A and Lopes da Silva, T. (2023) The Use of Oleaginous Yeasts and Microalgae Grown in Brewery Wastewater for Lipid Production and Nutrient Removal: A Review, Waste and Biomass Valorizati., 14, 1799–1822. https://doi.org/10.1007/s12649-023-02032-8

Dias, C., Nobre, B.P., Santos, J.A.L., Lopes da Silva, T.and Reis, A. (2022) Direct lipid and carotenoid extraction from Rhodosporidium toruloides broth culture after high pressure homogenization cell disruption: Strategies, methodologies, and yields, Biochem. Eng. J., 189, 108712. https:// doi.org/10.1016/j.bej.2022.108712

Izaguirre, J.K., Baranano, J., Castanón, S., Santos, J.A.L, Cesário, M.T., Fonseca, M.M.R., Alkorta, I.and Garbisu, C. (2021) Economic and environmental assessment of bacterial poly(3-hydroxybutyrate) production from the organic fraction of municipal solid waste, Bioresour. Bioprocess, 8, 39. https://doi.org/10.1186/s40643-021-00392-4

Marques S, Gírio FM, Roseiro JC, Santos JAL (2017) Lactic acid production from recycled paper sludge: process intensification by running fed-batch into a membrane-recycle bioreactor, Biochem. Eng. J., 120, 63–72, https://doi.org/10.1016/j.bej.2016.12.021

Prazeres, D.M.F. and J. A. L. Santos, A.L., Production and Purification of Adenovirus Vectors for Gene Therapy, in “Handbook of Pharmaceutical Biotechnology”, Shayne Cox Gad (Editor), John Wiley & Sons, Inc., 1262-1295, 2007, https://doi.org/10.1002/0470117117

E-mail (click on)

Alternative webpages
ResearchGate
CienciaID
Técnico Lisboa

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