Tiago André Melicias PitaTiago André Melicias Pita 



David Botequim
PhD Student (BIOTECnico Program)

Research Activities

Development of plasmon-enhanced fluorescence biosensors that explore the metal nanoparticles antenna effect for the detection of specific disease biomarkers., e. g. nucleic acids.
Functionalization of gold nanoparticles (rods, spheres, triangles) with nucleic acids for optical imaging and biosensing.
Synthesis and characterization of gold nanoparticles.

Selected Publications

Paulo P. M. R., Botequim D., Jóskowiak A., Martins S., Prazeres D. M. F., Zijlstra P., Costa S. M. B., “Enhanced Fluorescence of a Dye on DNA-Assembled Gold Nanodimers Discriminated by Lifetime Correlation Spectroscopy”, J. Phys. Chem. C, 122 (20), 10971–10980 (2018), DOI: 10.1021/acs.jpcc.7b12622.

Botequim D., Jóskowiak A., Prazeres D. M. F., Costa S. M. B., Paulo P M. R. “Gold Nanorods Functionalized with Fluorescently-Labeled DNA’s: a Single-Particle Spectroscopy Study”, 6 JIF – Jornadas Ibéricas de Fotoquímica, Aveiro, Portugal, 11-14 Setembro, 2018.

Botequim D., Prazeres D. M. F., Costa S. M. B., Paulo P M. R., “Gold Nanorods Conjugated with Fluorescently-Labeled DNA’s: Highly Loaded Dye-Nanoparticles”, Noble Metal Nanoparticles Gordon Research Conference, South Hadley, Massachusetts, USA, June 17 - 22, 2018.

Botequim D., Prazeres D. M. F., Costa S. M. B., Paulo P M. R., "Fluorescence Enhancement of Labeled Oligonucleotides Using Plasmonic Gold Nanorods Toward Biosensing Applications", nanoPT2018 International Conference, Lisboa, Portugal, 7-9 February, 2018.

Botequim D., Maia J., Lino M., Simões P., Ilharco L., Ferreira L., “Nanoparticles and surfaces presenting antifungal, antibacterial and antiviral properties”, Langmuir, 28 (20), 7647-7656 (2012), DOI: 10.1021/la300948n.


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