João Carlos Silva
PhD student

Research Activities

My research work is focused in the development of a new platform comprising 3D hierarchical scaffolds, stem cells and bioreactor prototypes for the production of native-like articular cartilage. This project will employ the use of different biomanufacturing techniques such as extrusion, hydrogel synthesis and electrospinning to produce scaffolds able to mimic the complex hierarchical structure of articular cartilage. Glycosaminoglycans (GAGs) known to have a role in the chondrogenic process will be studied and further incorporated within the scaffold structure. In addition, bioreactor prototypes able to provide different biophysical stimuli such as compressive loading and shear stress to promote chondrogenesis will be developed. We believe that our integrated strategy will play an important role in defining the optimal conditions for articular cartilage tissue engineering strategies.

Selected Publications

Aroso IM, Silva JC, Mano F, Sá-Nogueira I, Barreiros S, Reis RL, Paiva A, Duarte ARC (2016) Dissolution enhancement of active pharmaceutical ingredients by therapeutic deep eutectic systems, Eur. J. Pharm. Biopharm. 98: 57-66, doi: 10.1016/j.ejpb.2015.11.002

Silva JC, Barros AA, Aroso IM, Fassini D, Silva TH, Reis RL, Duarte ARC (2016) Collagen/Gelatin from Marine Demosponge Chondrosia reniformis (Nardo, 1847) using Water Acidified with Carbon Dioxide – Process Optimization, Ind. Eng. Chem. Res. 55: 6922-6930, doi: 10.1021/acs.iecr.6b00523

Silva JC, Moura CS, Viana T, Cabral JCS, Ferreira FC (2016) Poly (ε-caprolactone) scaffolds combined with extruded perfusion bioreactor prototypes as an integrated platform for articular cartilage tissue engineering, Eur. Cell. Mater. Suppl. 1: 101.

Silva JC, Moura CS, Alves NM, Cabral JCS, Ferreira FC (2017) Effects of different fibre alignments and bioactive coatings on mesenchymal stem/stromal cell adhesion and proliferation in poly (ε-caprolactone) scaffolds towards cartilage repair, Procedia. Eng. 12: 132-140. doi: 10.1016/j.promfg.2017.08.034

Silva JC, Moura CS, Borrecho G, Henriques P, Alves de Matos AP, Cabral JCS, Linhardt RL, Ferreira FC (2018) Mesenchymal stem cells combined with 3D extruded scaffolds and glycosaminoglycan supplementation as an integrated strategy towards cartilage repair: biochemical, structural and morphological analysis, Ann. Med. 50: Suppl. 51, doi: 10.1080/07853890.2018.1427445

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