João Carlos Silva
PhD student
Research Activities
My research work is focused in the development of a new platform comprising 3D hierarchical scaffolds, stem cells and bioreactor prototypes for the production of native-like articular cartilage. This project will employ the use of different biomanufacturing techniques such as extrusion, hydrogel synthesis and electrospinning to produce scaffolds able to mimic the complex hierarchical structure of articular cartilage. Glycosaminoglycans (GAGs) known to have a role in the chondrogenic process will be studied and further incorporated within the scaffold structure. In addition, bioreactor prototypes able to provide different biophysical stimuli such as compressive loading and shear stress to promote chondrogenesis will be developed. We believe that our integrated strategy will play an important role in defining the optimal conditions for articular cartilage tissue engineering strategies.
Selected Publications
Aroso IM, Silva JC, Mano F, Sá-Nogueira I, Barreiros S, Reis RL,
Paiva A, Duarte ARC (2016) Dissolution enhancement of active
pharmaceutical ingredients by therapeutic deep eutectic systems, Eur.
J. Pharm. Biopharm. 98: 57-66, doi: 10.1016/j.ejpb.2015.11.002
Silva JC, Barros AA, Aroso IM, Fassini D, Silva TH, Reis RL, Duarte ARC
(2016) Collagen/Gelatin from Marine Demosponge Chondrosia reniformis
(Nardo, 1847) using Water Acidified with Carbon Dioxide – Process
Optimization, Ind. Eng. Chem. Res. 55: 6922-6930, doi:
10.1021/acs.iecr.6b00523
Silva JC, Moura CS, Viana T, Cabral JCS, Ferreira FC (2016) Poly
(ε-caprolactone) scaffolds combined with extruded perfusion bioreactor
prototypes as an integrated platform for articular cartilage tissue
engineering, Eur. Cell. Mater. Suppl. 1: 101.
Silva JC, Moura CS, Alves NM, Cabral JCS, Ferreira FC (2017) Effects of
different fibre alignments and bioactive coatings on mesenchymal
stem/stromal cell adhesion and proliferation in poly (ε-caprolactone)
scaffolds towards cartilage repair, Procedia. Eng. 12: 132-140. doi:
10.1016/j.promfg.2017.08.034
Silva JC, Moura CS, Borrecho G, Henriques P, Alves de Matos AP, Cabral
JCS, Linhardt RL, Ferreira FC (2018) Mesenchymal stem cells combined
with 3D extruded scaffolds and glycosaminoglycan supplementation as an
integrated strategy towards cartilage repair: biochemical, structural
and morphological analysis, Ann. Med. 50: Suppl. 51, doi:
10.1080/07853890.2018.1427445
Alternative webpages
SCERG
ResearchGate