Sara Rosa
PhD Student

Research Activities

The overall goal of my research is to develop scalable, integrated and innovative downstream processes for the purification of monoclonal antibodies (mAbs) based on clarification (cell harvest) by extraction using aqueous two-phase systems, and further purification by multimodal chromatographic systems operated under axial and/or radial modes. The thermodynamics of mAbs interaction with the different chromatography ligands and polymers are also of great interest in my research. I’m also focused in the optimization of the production of mAbs using mammalian cell technology.

Selected Publications

Rosa SASL, da Silva CL, Aires-Barros MR, Dias-Cabral AC, Azevedo AM (2018). Thermodynamics of the adsorption of monoclonal antibodies in phenylboronate chromatography: Affinity versus multimodal interactions. J Chromatogr A 118-127, doi: 10.1016/j.chroma.2018.07.050.

Rosa SASL*, dos Santos R*, Aires-Barros MR, Azevedo AM (2016). Phenylboronic acid chromatography provides a rapid, reproducible and easy scalable multimodal process for the capture of monoclonal antibodies. Sep Purif Technol 160: 43-50, doi: 10.1016/j.seppur.2016.01.002. *The authors contributed equally.

dos Santos R*, Rosa SASL*, Aires-Barros MR, Tover A, Azevedo AM (2014). Phenylboronic acid as a multi-modal ligand for the capture of monoclonal antibodies: development and optimization of a washing step. J Chromatogr A 1355: 115-124, doi: 10.1016/j.chroma.2014.06.001. *The authors contributed equally.

Campos-Pinto I, Espitia-Saloma E, Rosa SASL, Rito-Palomares M, Aguilar O, Arévalo-Rodríguez M, Aires-Barros MR, Azevedo AM (2017). Integration of cell harvest with affinity-enhanced purification of monoclonal antibodies using aqueous two-phase systems with a dual tag ligand. Sep Purif Technol 173: 129-134, doi: 10.1016/j.seppur.2016.09.017.

Pinto IF, Soares RRG, Rosa SASL, Aires-Barros MR, Chu V, Conde JP, Azevedo, AM (2016). High-throughput nanoliter-scale analysis and optimization of multimodal chromatography for the capture of monoclonal antibodies. Anal Chem 88(16): 7959-7967, doi: 10.1021/acs.analchem.6b00781.

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